Frequently Asked Questions: Custom Annotation Tracks
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Displaying personal annotation data in the Genome Browser |
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Question:
"How do I display my own personal annotation data in the Genome Browser?"
Response:
To create an annotation track that will display on the
Genome Browser, you must first organize your data into
one of the formats supported by the
browser custom track feature:
GTF,
GFF,
BED,
WIG,
or PSL.
Then, upload your data into the Genome Browser on
the Add Custom Tracks
page. Once you've created your annotation track, you can
share it with others over the internet by putting your
annotation file on your website, then creating a custom
URL that allows others to directly start the browser
with your track displayed.
Read the Creating custom annotation tracks
section in the Genome Browser User's Guide for
a step-by-step description of how to format and display
a custom annotation track and create a custom URL.
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Adding a personal annotation track to the Genome Browser website |
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Question:
"I have an annotation track that I think might be of general interest to the
research community. Will UCSC consider including my track in their browser?"
Response:
We are always interested in receiving new annotation tracks for the Genome Browser, and
encourage our users to share their tracks with us and others in the research
community. Please send the URL for your track -- along with a description of the
methods, data, and format used -- to
genome@soe.ucsc.edu.
If we determine that your track is of sufficient general interest to distribute as part of our browser, we'll work
with you to include it.
In addition to the standard set of tracks displayed on the Genome Browser page, we
also have a Custom
Annotation Tracks page that contains contributed tracks
we are unable to display in the main browser (for example, tracks that are of too
specific an interest or are too sparse). We welcome contributions to this page.
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Defining filter parameters for custom tracks |
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Question:
"Is it possible to define a filter parameter for a custom track to
highlight certain features?"
Response:
This feature is not currently implemented.
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Coloring a custom track using the useScore parameter |
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Question:
"When designing a custom track, is there a way to assign specific colors
to each segment, as is done in the mouse/rat synteny tracks? Is there a way
to assign
a value range for the useScore variable such that I can have four shades
and specify the value range for each?"
Response:
Currently useScore works only with tracks that are black or specific shades of
brown or blue. The score range is 0-1000. To display four shades, use the scores 0, 333, 666,
and 1000.
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Constructing a Genome Browser URL |
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Question:
"How can I construct a URL to retrieve data from the Genome Browser?
What do the various parameters in the Genome Browser URLs mean?"
Response:
One way to determine how to construct a correct URL is to open a Genome Browser link
in which you are interested and examine how the Genome Browser constructs the URL.
See the User's Guide
for a discussion of the basic
components of a Genome Browser URL. Note that the c parameter that appears
in some URLs specifies the chromosome name or the chromosome name and position.
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Using the hgsid parameter in a URL |
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Question:
"Should I use the hgsid parameter in my URL?"
Response:
Avoid using hgsid -- it is a temporary identifier,
and will typically stop working after a day.
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Creating a details page for a custom annotation track |
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Question:
"While working on a custom track, we noticed that the feature details page
for custom track looks different from regular tracks on the site. Is the
details page for a custom track customizable?"
Response:
"The details page for the a custom track can be submitted by pasting the
text in the "optional track documentation" box, uploading from a file,
or via URL by specifying the 'htmlUrl' attribute to the track line of
your custom track. This can be done either from the Add Custom Tracks
page or from the Update Custom Tracks page."
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Custom annotation track troubleshooting |
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Question:
"When I click the Submit button, I get the error message
"line # of custom input: BED chromStarts[i] must be in ascending order"."
Response:
We have tightened up the input checking on custom tracks. This error
is caused by a logical conflict in the Genome Browser software. It
accepts custom GFF tracks that have multiple "exons" at the
same position, but not BED tracks that have this feature. Because the
browser translates GFF tracks to BED format before storing the custom
track data, GFF tracks with multiple exons will cause an error when
the BED is read back in. To work around this problem, remove duplicate
lines in the GFF track.
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